Table 2

Test media recipes as defined by ASTM and ISO standards

SerumSodium azide (%) (anti-microbial)EDTA (mM)Temperature (°C)pH
ASTM 1714 Hip48 Filtered bovine Blood serum0.2*2037±3
ASTM 1715 Knee49 Filtered bovine Blood serum0.2 to 0.3*2021 to 39 (±3)
ASTM F2423-05 Disc11 Bovine serum, 20 g/L0.2*2037±3
ISO 14242 Hip50 Filtered (2 µm) Calf serum, not less than 17 g/LMay be added*37±2Monitor
ISO 14243 Knee51 Filtered (2 µm) Calf serum, not less than 17 g/LMay be added*37±2Monitor
ISO 18192-1 Disc10 Filtered (2 µm) Calf serum, 30±2 g/LMay be added*2037±2Monitor
  • * Or other suitable antibiotic.