Abstract
Amniotic membrane (AM) or amnion is a thin membrane on the inner side of the fetal placenta; it completely surrounds the embryo and delimits the amniotic cavity, which is filled by amniotic liquid. In recent years, the structure and function of the amnion have been investigated, particularly the pluripotent properties of AM cells, which are an attractive source for tissue transplantation. AM has anti-inflammatory, anti-bacterial, anti-viral and immunological characteristics, as well as anti-angiogenic and pro-apoptotic features. AM is a promoter of epithelialization and is a non-tumorigenic tissue and its use has no ethical problems. Because of its attractive properties, AM has been applied in several surgical procedures related to ocular surface reconstruction and the genito-urinary tract, skin, head and neck, among others. So far, the best known and most auspicious applications of AM are ocular surface reconstruction, skin applications and tissue engineering. However, AM can also be applied in oncology. In this area, AM can prevent the delivery of nutrients and oxygen to cancer cells and consequently interfere with tumour angiogenesis, growth and metastasis.
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Adinolfi M, Akle C, McColl I, Fensom A, Tansley L, Connolly P, His B, Faulk W, Travers P, Bodmer W (1982) Expression of HLA antigens, [beta]2-microglobulin and enzymes by human amniotic epithelial cells. Nature 295:325–327
Akashi T, Miyagi T, Ando N, Suzuki Y, Nemoto T, Eishi Y, Nakamura K, Shirasawa T, Osa N, Tanaka N, Burgeson R (1999) Synthesis of basement membrane by gastrointestinal cancer cell lines. J Pathol 187:223–228
Akle C, Welsh K, Adinolfi M, Leibowitz S, Mccoll I (1981) Immunogenicity of human amniotic epithelial cells after transplantation into volunteers. Lancet 318:1003–1005
Amer M, Abd-El-Maeboud K (2006) Amnion graft following hysteroscopic lysis of intrauterine adhesions. J Obstet Gynaecol Res 32:559–566
Baradaran-Rafii A, Arjmand B, Javadi M (2007) Amniotic membrane transplantation. Iran J Ophthal Res 2:58–75
Bari M, Choudhury M, Khan A, Nessa A (2002) Role of human fetal membranes (amniotic membrane) in the management of burn wounds. Ann Burn Fire Disasters 15:12–16
Benedetti WL, Sala MA, Alvarez H (1973) Histochemical demonstration of enzymes in the umbilical cord and membranes of human term pregnancy. Eur J Obstet Gynecol Reprod Biol 3:185–189
Benedetto M, De Cicco F, Rossiello F, Nicosia A, Lupi G, Dell Acqua S (1990) Oxytocin receptor in human fetal membranes at term and during labor. J Steroid Biochem 35:205–208
Benirschke K (2000) Pathology of the human placenta. Springer, New York
Boudreau N, Sympson C, Werb Z, Bissell M (1995) Suppression of ICE and apoptosis in mammary epithelial cells by extracellular matrix. Science 267:891–893
Bourne G (1960) The microscopic anatomy of the human amnion and chorion. Am J Obstet Gynecol 79:1070–1073
Bryant-Greenwood G, Rees M, Turnbull A (1987) Immunohistochemical localization of relaxin, prolactin and prostaglandin synthase in human amnion, chorion and decidua. J Endocrinol 114:491–496
Buhimschi I, Jabr M, Buhimschi C, Petkova A, Weiner C, Saed G (2004) The novel antimicrobial peptide β3- defensin is produced by the amnion: a possible role of the fetal membranes in innate immunity of the amniotic cavity. Am J Obstet Gynecol 191:1678–1687
Burman S, Tejwani S, Vemuganti G, Gopinathan U, Sangwan V (2004) Ophthalmic applications of preserved human amniotic membrane: a review of current indications. Cell Tissue Bank 5:161–175
Bussink J, Kaander J, Kogel A van der (2003) Tumor hypoxia at the micro-regional level: clinical relevance and predictive value of exogenous and endogenous hypoxic cell markers. Radiother Oncol 67:3–15
Capeáns C, Piñeiro A, Pardo M, Sueiro-López C, Blanco M, Domínguez F, Sánchez-Salorio M (2003) Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Acta Ophthalmol Scand 81:271–277
Cheung P, Walton J, Tai H, Riley S, Challis J (1990) Immunocytochemical distribution and localization of 15- hydroxyprostaglandin dehydrogenase in human fetal membranes, decidua, and placenta. Am J Obstet Gynecol 163:1445–1449
Choi T, Tseng S (2001) In vivo and in vitro demonstration of epithelial cell-induced myofibroblast differentiation of keratocytes and an inhibitory effect by amniotic membrane. Cornea 20:197–204
Cooper G, Hausman R (2003) The cell: a molecular approach. American Socity for Microbiology, Washington
Crescimanno C (1993) Immunocytochemical patterns of carbonic anhydrase isoenzymes in human placenta, cord and membranes. Placenta 14:A11
Cunningham F (2001) Williams obstetrics. Slock, London
Danforth D, Hull R (1958) The microscopic anatomy of the fetal membranes with particular reference to the detailed structure of the amnion. Am J Obstet Gynecol 75:536–547
Davis J (1910) Skin transplantation with a review of 550 cases at the Johns Hopkins Hospital. Johns Hopkins Med J 15:307
Dua H, Gomes J, King A, Maharajan V (2004) The amniotic membrane in ophthalmology. Surv Ophthalmol 49:51–77
Enders A, King B (1988) Formation and differentiation of extraembryonic mesoderm in the rhesus monkey. Am J Anat 181:327–340
Fatima A, Balasubramanian D, Iftekhar G, Vemuganti G, Matalia H, Reddy P, Sangwan V (2006) Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation. J Postgrad Med 52:257–261
Fernandes M, Sridhar M, Sangwan V, Rao G (2005) Amniotic membrane transplantation for ocular surface reconstruction. Cornea 24:643–653
Fukuda K, Chikama T, Nakamura M, Nishida T (1999) Differential distribution of subchains of the basement membrane components type IV collagen and laminin among the AM, cornea, and conjunctiva. Cornea 18:73–79
Gibb W, Lavoie J (1990) Effects of glucocorticoids on prostaglandin formation by human amnion. Can J Physiol Pharmacol 68:671–676
Gomes J, Romano A, Santos M, Dua H (2005) Amniotic membrane use in ophthalmology. Curr Opin Ophthalmol 16:233–240
Guo M, Grinnell F (1989) Basement membrane and human epidermal differentiation in vitro. J Invest Dermatol 93:372–378
Hajiiski O (1990) Amniotic membranes for temporary burn coverage. Ann Burn Fire Disasters 9:88–92
Hammer A, Hutter H, Blaschitz A, Mahnert W, Hartmann M, Uchanska-Ziegler B, Ziegler A, Dohr G (1997) Amnion epithelial cells, in contrast to trophoblast cells, express all classical HLA class I molecules together with HLA-G. Am J Reprod Immunol 37:161–171
Hanahan D, Weinberg R (2000) The hallmarks of cancer. Cell 100:57–70
Hao Y, Ma D, Hwang D, Kim W, Zhang F (2000) Identification of antiangiogenic and antiinflammatory proteins in human AM. Cornea 19:348–352
Harder J, Meyer-Hoffert U, Teran L, Schwichtenberg L, Bartels J, Maune S, Schroder J (2000) Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am J Respir Cell Mol Biol 22:714–721
Herendael B van, Oberti C, Brosens I (1978) Microanatomy of the human amniotic membranes. A light microscopic, transmission, and scanning electron microscopic study. Am J Obstet Gynecol 131:872–880
Higa K, Shimmura S, Shimazaki J, Tsubota K (2005) Hyaluronic acid-CD44 interaction mediates the adhesion of lymphocytes by amniotic membrane stroma. Cornea 24:206–212
Houlihan J, Biro P, Harper H, Jenkinson H, Holmes C (1995) The human amnion is a site of MHC class Ib expression: evidence for the expression of HLA-E and HLA-G. J Immunol 154:5665–5674
Ishino Y, Sano Y, Nakamura T, Connon C, Rigby H, Fullwood N, Kinoshita S (2004) Amniotic membrane as a carrier for cultivated human corneal endothelial cell transplantation. Invest Ophthalmol Vis Sci 45:800–806
Jin C, Park S, Choi B, Lee K, Kang C, Min B (2007) Human amniotic membrane as a delivery matrix for articular cartilage repair. Tissue Eng 13:693–702
Jones S, Challis J (1989) Local stimulation of prostaglandin production by corticotropin-releasing hormone in human fetal membranes and placenta. Biochem Biophys Res Commun 159:192–199
Kakishita K, Elwan M, Nakao N, Itakura T, Sakuragawa N (2000) Human amniotic epithelial cells produce dopamine and survive after implantation into the striatum of a rat model of Parkinson’s disease: a potential source of donor for transplantation therapy. Exp Neurol 165:27–34
Kamiya K, Wang M, Uchida S, Amano S, Oshika T, Sakuragawa N, Hori J (2005) Topical application of culture supernatant from human amniotic epithelial cells suppresses inflammatory reactions in cornea. Exp Eye Res 80:671–679
Kaneko Y, Sakakibara S, Imai T, Suzuki A, Nakamura Y, Sawamoto K, Ogawa Y, Toyamad Y, Miyata T, Okano H (2000) Musashi1: an evolutionally conserved marker for CNS progenitor cells including neural stem cells. Dev Neurosci 22:139–153
Kanyshkova T, Buneva V, Nevinsky G (2001) Lactoferrin and its biological functions. Biochemistry (Mosc) 66:1–7
Kasahara H, Usheva A, Ueyama T, Aoki H, Horikoshi N, Izumo S (2001) Characterization of homo- and heterodimerization of cardiac Csx/Nkx2.5 homeoprotein. J Biol Chem 276:4570–4580
Keelan J, Sato T, Mitchell M (1997) Interleukin (IL)-6 and IL-8 production by human amnion: regulation by cytokines, growth factors, glucocorticoids, phorbol esters, and bacterial lipopolysaccharide. Biol Reprod 57:1438–1444
Keelan J, Sato T, Hansen W, Gilmour J, Gupta D, Helsby N, Mitchell M (1999) Interleukin-4 differentially regulates prostaglandin production in amnion-derived WISH cells stimulated with pro-inflammatory cytokines and epidermal growth factor. Prostaglandins Leukot Essent Fatty Acids 60:255–262
Khouw I, Wachem P van, Plantinga J, Vujaskovic Z, Wissink M, Leij L de, Luyn M van (1999) TGF-[beta] and bFGF affect the differentiation of proliferating porcine fibroblasts into myofibroblasts in vitro. Biomaterials 20:1815–1822
Kim J, Tseng S (1995a) The effects on inhibition of corneal neovascularization after human AM transplantation in severely damaged rabbit corneas. Korean J Ophthalmol 9:32–46
Kim J, Tseng S (1995b) Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea 14:473–484
Kim J, Kim J, Na B, Jeong J, Song C (2000) Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkali burn. Exp Eye Res 70:329–337
King A, Critchley H, Sallenave J, Kelly R (2003) Elafin in human endometrium: an antiprotease and antimicrobial molecule expressed during menstruation. J Clin Endocrinol Metab 88:4426–4431
King A, Paltoo A, Kelly R, Sallenave J, Bocking A, Challis J (2007) Expression of natural antimicrobials by human placenta and fetal membranes. Placenta 28:161–169
King B (1985) Related distribution and characterization of anionic sites in the basal lamina of developing human amniotic epithelium. Anat Rec 212:57–62
Knudson G (1993) Antioncogenes and human cancer. Proc Natl Acad Sci USA 90:10914–10921
Kobayashi N, Kabuyama Y, Sasaki S, Kato K, Homma Y (2002) Suppression of corneal neovascularization by culture supernatant of human amniotic cells. Cornea 21:62–67
Koizumi N, Inatomi T, Sotozono C, Fullwood N, Quantock A, Kinoshita S (2000) Growth factor mRNA and protein in preserved human amniotic membrane. Curr Eye Res 20:173–177
Koyano S, Fukui A, Uchida S, Yamada K, Asashima M, Sakuragawa N (2002) Synthesis and release of activin and noggin by cultured human amniotic epithelial cells. Dev Growth Differ 44:103–112
Krisanaprakornkit S, Weinberg A, Perez C, Dale B (1998) Expression of the peptide antibiotic human beta- defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun 66:4222–4228
Kubo M, Sonoda Y, Muramatsu R, Usui M (2001) Immunogenicity of human amniotic membrane in experimental xenotransplantation. Invest Ophthalmol Vis Sci 42:1539–1546
Lee S, Tseng S (1997) Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol 123:303–312
Lee S, Li D, Tan D, Meller D, Tseng S (2000) Suppression of TGF-beta signaling in both normal conjunctival fibroblasts and pterygial body fibroblasts by amniotic membrane. Curr Eye Res 20:325–334
Li H, Niederkorn J, Neelam S, Mayhew E, Word R, McCulley J, Alizadeh H (2005) Immunosuppressive factors secreted by human amniotic epithelial cells. Invest Ophthalmol Vis Sci 46:900–907
Li W, He H, Kawakita T, Espana E, Tseng S (2006) Amniotic membrane induces apoptosis of interferon-[gamma] activated macrophages in vitro. Exp Eye Res 82:282–292
Mahgoub M, Ammar A, Fayez M, Edris A, Hazem A, Akl M, Hammam O (2004) Neovascularization of the amniotic membrane as a biological immune barrier. Transplant Proc 36:1194–1198
Mencucci R, Paladini I, Menchini U, Gicquel J, Dei R (2011) Inhibition of viral replication in vitro by antiviral-treated amniotic membrane. Possible use of amniotic membrane as drug-delivering tool. Br J Ophthalmol 95:28–31
Mermet I, Pottier N, Sainthillier J, Malugani C, Cairey-Remonnay S, Maddens S, Riethmuller D, Tiberghien P, Humbert P, Aubin F (2007) Use of amniotic membrane transplantation in the treatment of venous leg ulcers. Wound Repair Regen 15:459–464
Miki T, Lehmann T, Cai H, Stolz D, Strom S (2005) Stem cell characteristics of amniotic epithelial cells. Stem Cells 23:1549–1559
Miller J, Michel J, Bercovici B, Argaman M, Sacks T (1976) Studies on the antimicrobial activity of amniotic fluid. Am J Obstet Gynecol 125:212–214
Mligiliche N, Endo K, Okamoto K, Fujimoto E, Ide C (2002) Extracellular matrix of human amnion manufactured into tubes as conduits for peripheral nerve regeneration. J Biomed Mater Res 63:591–600
Mohammad J, Shenaq J, Rabinovsky E, Shenaq S (2000) Modulation of peripheral nerve regeneration: a tissue-engineering approach. The role of amnion tube nerve conduit across a 1-centimeter nerve gap. Plast Reconstr Surg 105:660–666
Muhlhauser J, Crescimanno C, Rajaniemi H, Parkkila S, Milovanov A, Castellucci M, Kaufmann P (1994) Immunohistochemistry of carbonic anhydrase in human placenta and fetal membranes. Histochemistry 101:91–98
Nakajima T, Enosawa S, Mitani T, Li X, Suzuki S, Amemiya H, Koiwai O, Sakuragawa N (2001) Cytological examination of rat amniotic epithelial cells and cell transplantation to the liver. Cell Transplant 10:423–427
Ni J, Abrahamson M, Zhang M, Fernandez M, Grubb A, Su J, Yu G, Li Y, Parmelee D, Xing L, Coleman T, Gentz S, Thotakura R, Nguyen N, Hesselberg M, Gentz R (1997) Cystatin E is a novel human cysteine proteinase inhibitor with structural resemblance to family 2 cystatins. J Biol Chem 272:10853–10858
Niknejad H, Peirovi H, Jorjani M, Ahmadiani A, Ghanavi J, Seifalian A (2008) Properties of the amniotic membrane for potential use in tissue engineering. Eur Cell Mater 15:88–99
Nishimura W, Kondo T, Salameh T, El Khattabi I, Dodge R, Bonner-Weir S, Sharma A (2006) A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells. Dev Biol 293:526–539
Ochsenbein-Kölble N, Jani J, Lewi L, Verbist G, Vercruysse L, Portmann-Lanz B, Marquardt K, Zimmermann R, Deprest J (2007) Enhancing sealing of fetal membrane defects using tissue engineered native amniotic scaffolds in the rabbit model. Am J Obstet Gynecol 196:263.e1-263.e7
Ogawa A, Terada S, Sakuragawa N, Masuda S, Nagao M, Miki M (2003) Progesterone, but not 17beta-estradiol, up-regulates erythropoietin (EPO) production in human amniotic epithelial cells. J Biosci Bioeng 96:448–453
Okazaki T, Casey M, Okita J, MacDonald P, Johnston J (1981) Initiation of human parturition. XII. Biosynthesis and metabolism of prostaglandins in human fetal membranes and uterine decidua. Am J Obstet Gynecol 139:373–381
Pabuçcu R, Atay V, Orhon E, Urman B, Ergün A (1997) Hysteroscopic treatment of intrauterine adhesions is safe and effective in the restoration of normal menstruation and fertility. Fertil Steril 68:1141–1143
Park W, Tseng S (2000) Modulation of acute inflammation and keratocyte death by suturing, blood, and amniotic membrane in PRK. Invest Ophthalmol Vis Sci 41:2906–2914
Parry S, Strauss J (1998) Premature rupture of the fetal membranes. N Engl J Med 338:663–670
Perera F, Weinstein I (2000) Molecular epidemiology: recent advances and future directions. Carcinogenesis 21:517–524
Pollard S, Aye N, Symonds E (1976) Scanning electron microscope appearances of normal human amnion and umbilical cord at term. Br J Obstet Gynaecol 83:470–477
Portmann-Lanz C, Ochsenbein-Kölble N, Marquardt K, Lüthi U, Zisch A, Zimmermann R (2007) Manufacture of a cell-free amnion matrix scaffold that supports amnion cell outgrowth in vitro. Placenta 28:6–13
Robinson W, McFadden D, Barrett I, Kuchinka B, Peñaherrera M, Bruyère H, Best R, Pedreira D, Langlois S, Kalousek D (2002) Origin of amnion and implications for evaluation of the fetal genotype in cases of mosaicism. Prenat Diagn 22:1076–1085
Rote N (1993) Expression of IL-1 and IL-6 protein and mrna in amniochorionic membranes. Placenta 14:A63
Rotth A (1940) Plastic repair of conjunctival defects with fetal membranes. Arch Ophthalmol 23:522–525
Rukstalis J, Ubeda M, Johnson M, Habener J (2006) Transcription factor snail modulates hormone expression in established endocrine pancreatic cell lines. Endocrinology 147:2997–3006
Runić R, Lockwood C, LaChapelle L, Dipasquale B, Demopoulos R, Kumar A, Guller S (1998) Apoptosis and Fas expression in human fetal membranes. J Clin Endocrinol Metab 83:660–666
Sabella N (1913) Use of the fetal membranes in skin grafting. Med Rec 83:478–480
Sadler T (2000) Langmans medical embryology. Slock, London
Sakuragawa N, Yoshikawa H, Sasaki M (1992) Amniotic tissue transplantation: clinical and biochemical evaluations for some lysosomal storage diseases. Brain Dev 14:7–11
Sakuragawaa N, Misawab H, Ohsugia K, Kakishitaa K, Ishiia T, Thangavela R, Tohyamaa J, Elwana M, Yokoyamac Y, Okudaa O, Araia H, Oginod I, Sato K (1997) Evidence for active acetylcholine metabolism in human amniotic epithelial cells: applicable to intracerebral allografting for neurologic disease. Neurosci Lett 232:53–56
Scaggiante B, Pineschi A, Sustersich M, Andolina M, Agosti E, Romeo D (1987) Successful therapy of Niemann-Pick disease by implantation of human amniotic membrane. Transplantation 44:59–61
Seo J, Kim Y, Kim J (2008) Properties of the amniotic membrane may be applicable in cancer therapy. Med Hypotheses 70:812–814
Shao C (2004) Suppression of corneal neovascularization by PEDF release from human amniotic membranes. Invest Ophthalmol Vis Sci 45:1758–1762
Shimazaki J, Shinozaki N, Tsubota K (1998) Transplantation of amniotic membrane and limbal autograft for patients with recurrent pterygium associated with symblepharon. Br J Ophthalmol 82:235–240
Shimmura S, Shimazaki J, Ohashi Y, Tsubota K (2001) Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders. Cornea 20:408–413
Shumway J, Al-Malt A, Amon E, Cohlan B, Amini S, Abboud M, Winn H (1999) Impact of oligohydramnios on maternal and perinatal outcomes of spontaneous premature rupture of the membranes at 18–28 weeks. J Matern Fetal Med 8:20–23
Sorsby A, Haythorne J, Reed H (1947) Further experience with amniotic membrane grafts in caustic burns of the eye. Br J Ophthalmol 31:409–418
Spicer S, Schulte B (1998) Evidence for a medial K+ recycling pathway from inner hair cells. Hear Res 118:1–12
Stern M (1913) The grafting of preserved amniotic membranes to burned and ulcerated surfaces, substituting skin grafts. JAMA 60:973
Takashima S, Ise H, Zhao P, Akaike T, Nikaido T (2004) Human amniotic epithelial cells possess hepatocyte-like characteristics and functions. Cell Struct Funct 29:73–84
Takashima S, Yasuo M, Sanzen N, Sekiguchi K, Okabe M, Yoshida T, Toda A, Nikaido T (2008) Characterization of laminin isoforms in human amnion. Tissue Cell 40:75–81
Tanaka M, Chen Z, Bartunkova S, Yamasaki N, Izumo S (1999) The cardiac homeobox gene Csx/Nkx2.5 lies genetically upstream of multiple genes essential for heart development. Development 126:1269–1280
Thadepalli H, Bach V, Davidson E (1978) Antimicrobial effect of amniotic fluid. Obstet Gynecol 52:198–204
Toda A, Okabe M, Yoshida T, Nikaido T (2007) The potential of amniotic membrane/amnion-derived cells for regeneration of various tissues. J Pharmacol Sci 105:215–228
Toth P (1992a) Expression of hcg/lh receptor gene and its functional coupling to the regulation of cyclooxygenase-1 and −2 enzymes in human fetal membranes. Placenta 14:A78
Toth P (1992b) Direct novel regulation of cyclooxygenase (cox) and prostacyclin synthase (pgi2-s) by hCG in human amnion. Placenta 13:A63
Toth P, Li X, Lei Z, Rao C (1996) Expression of human chorionic gonadotropin (hCG)/luteinizing hormone receptors and regulation of the cyclooxygenase-1 gene by exogenous hCG in human fetal membranes. J Clin Endocrinol Metab 81:1283–1288
Tsai S, Liu Y, Tang W, Zhou Z, Hwang C, Hwang G, Ou B, Hu C, Yang V, Chen J (2007) Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering. Biochem Biophys Res Commun 357:984–990
Tseng S, Prabhasawat P, Lee S (1997) Amniotic membrane transplantation for conjunctival surface reconstruction. Am J Ophthalmol 124:765–774
Tseng S, Li D, Ma X (1999) Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix. J Cell Physiol 179:325–335
Uchida S, Inanaga Y, Kobayashi M, Hurukawa S, Araie M, Sakuragawa N (2000) Neurotrophic function of conditioned medium from human amniotic epithelial cells. J Neurosci Res 62:585–590
Wang J (2004) The concerted activities of Pax4 and Nkx2.2 are essential to initiate pancreatic β-cell differentiation. Dev Biol 266:178–189
Wei J, Zhang T, Kawa S, Aizawa T, Ota M, Akaike T, Kato K, Konishi I, Nikaido T (2003) Human amnion-isolated cells normalize blood glucose in streptozotocin-induced diabetic mice. Cell Transplant 12:545–552
Weinberg R (1991) Tumor suppressor genes. Science 254:1138–1146
Wolf H, Desoye G (1993) Immunohistochemical localization of glucose transporters and insulin receptors in human fetal membranes at term. Histochemistry 100:379–385
Wolf H, Schmidt W, Drenckhahn D (1991) Immunocytochemical analysis of the cytoskeleton of the human amniotic epithelium. Cell Tissue Res 266:385–389
Yang L, Shirakata Y, Shudou M, Dai X, Tokumaru S, Hirakawa S, Sayama K, Hamuro J, Hashimoto K (2006) New skin-equivalent model from de-epithelialized amnion membrane. Cell Tissue Res 326:69–77
Yeh L, Chen W, Li W, Espana E, Ouyang J, Kawakita T, Kao W, Tseng S, Liu C (2005) Soluble lumican glycoprotein purified from human amniotic membrane promotes corneal epithelial wound healing. Invest Ophthalmol Vis Sci 46:479–486
Yu J, Zhang L (2004) Apoptosis in human cancer cells. Curr Opin Oncol 16:19–24
Yuge I, Takumi Y, Koyabu K, Hashimoto S, Takashima S, Fukuyama T, Nikaido T, Usami S (2004) Transplanted human amniotic epithelial cells express connexin 26 and Na-K-adenosine triphosphatase in the inner ear. Transplantation 77:1452–1454
Zhou S, Chen J, Feng J (2003) The effects of amniotic membrane on polymorphonuclear cells. Chin Med J 116:788–790
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Mamede, A.C., Carvalho, M.J., Abrantes, A.M. et al. Amniotic membrane: from structure and functions to clinical applications. Cell Tissue Res 349, 447–458 (2012). https://doi.org/10.1007/s00441-012-1424-6
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DOI: https://doi.org/10.1007/s00441-012-1424-6