An in vitro biomechanical study was conducted to determine the effects of fusion and nonfusion anterior cervical instrumentation on cervical spine biomechanics in a multilevel human cadaveric model. Three spine conditions were studied: harvested, single-level artificial cervical joint, and single-level graft with anterior cervical plate. A programmable testing apparatus was used that replicated physiologic flexion/extension and lateral bending. Measurements included vertebral motion, applied load, and bending moments. Relative rotations at the superior, implanted, and inferior motion segment units (MSUs) were normalized with respect to the overall rotation of those three MSUs and compared using a one-way analysis of variance (P < 0.05). Application of an anterior cervical plate decreased motion across the fusion site relative to the harvested and artificial joint spine conditions. The reduced motion was compensated for by an increase in motion at the adjacent segments. Use of an artificial cervical joint did not alter the motion patterns at either the instrumented level or the adjacent segments compared with the harvested condition for all modes of testing.