To date, there are only a few studies that provide data to efficiently calibrate finite element models for the spine due to its complexity. In a recent study, we quantified the range of motion rotation and the lordosis angle. This paper provides complementary results regarding two more parameters, intradiscal pressure and vertebral translation. All parameters were obtained as a function of stepwise anatomical reduction, loading direction and magnitude. Eight lumbar spinal segments (L4-5) with a median age of 52 years (38-59 years) and no signs of disc degeneration were used for the in vitro testing. A miniaturized pressure probe was implanted into the nucleus. An ultrasound-based motion-tracking system was employed to record spatial movements of several landmarks on the specimens. The center of L4, the anterior, posterior, left and right point of the lower endplate of L4 were digitized as landmarks and its translation was determined. Specimens were loaded with pure moments (1-10Nm) in the three principal anatomical planes at a loading rate of 1.0 degrees /s. Anatomy was stepwise reduced by cutting different ligaments, facet capsules and joints and removing nucleus. Translation analysis showed that the L4 center point had its largest displacement in sagittal direction and almost none vertically. Removal of the supra- and interspinous, flaval ligaments showed a slight increase and further removal of structures, a higher increase of translation. Axial rotation also was accompanied with L4 to elevate when torsion was applied. This effect was found to be larger with progressing defects. Nucleotomy exhibited the most unstable situation for specimens. Results of the intradiscal pressure indicated a large increase after removing the facet capsules and joints. Furthermore, it was found that intradiscal pressure correlated well with data of range of motion for rotation. Predicting and simulating clinical defects, surgical intervention or treatment methods requires a well performed calibration based on in vitro data, whereas it is important to adapt all including structures with as many known parameters as possible. Results provided by these studies may be used as a database for researchers aiming to calibrate or validate finite element models of L4-5 segments.