p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

…, C Russ, J Reece-Hoyes, W Forrester, GR Hoffman… - Nature medicine, 2018 - nature.com
CRISPR/Cas9 has revolutionized our ability to engineer genomes and conduct genome-wide
screens in human cells 1 , 2 – 3 . Whereas some cell types are amenable to genome …

Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling

…, Q Yang, XM Ma, J Villén, N Kubica, GR Hoffman… - Science, 2011 - science.org
The evolutionarily conserved serine-threonine kinase mammalian target of rapamycin (mTOR)
plays a critical role in regulating many pathophysiological processes. Functional …

[HTML][HTML] Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI

GR Hoffman, N Nassar, RA Cerione - Cell, 2000 - cell.com
The RhoGDI proteins serve as key multifunctional regulators of Rho family GTP-binding
proteins. The 2.6 Ċ X-ray crystallographic structure of the Cdc42/RhoGDI complex reveals two …

[PDF][PDF] Project DRIVE: a compendium of cancer dependencies and synthetic lethal relationships uncovered by large-scale, deep RNAi screening

…, MR Schlabach, E Billy, KJ Mavrakis, GR Hoffman… - Cell, 2017 - cell.com
Elucidation of the mutational landscape of human cancer has progressed rapidly and been
accompanied by the development of therapeutics targeting mutant oncogenes. However, a …

[PDF][PDF] DNA repair profiling reveals nonrandom outcomes at Cas9-mediated breaks

…, C Nye, S Gradia, B Vidal, J Zheng, GR Hoffman… - Molecular cell, 2016 - cell.com
The repair outcomes at site-specific DNA double-strand breaks (DSBs) generated by the
RNA-guided DNA endonuclease Cas9 determine how gene function is altered. Despite the …

Functional epigenetics approach identifies BRM/SMARCA2 as a critical synthetic lethal target in BRG1-deficient cancers

GR Hoffman, R Rahal, F Buxton… - Proceedings of the …, 2014 - National Acad Sciences
Defects in epigenetic regulation play a fundamental role in the development of cancer, and
epigenetic regulators have recently emerged as promising therapeutic candidates. We …

Disordered methionine metabolism in MTAP/CDKN2A-deleted cancers leads to dependence on PRMT5

…, ER McDonald III, MR Schlabach, E Billy, GR Hoffman… - Science, 2016 - science.org
5-Methylthioadenosine phosphorylase (MTAP) is a key enzyme in the methionine salvage
pathway. The MTAP gene is frequently deleted in human cancers because of its chromosomal …

[PDF][PDF] Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex

SG Kim, GR Hoffman, G Poulogiannis, GR Buel… - Molecular cell, 2013 - cell.com
The metabolism of glucose and glutamine, primary carbon sources utilized by mitochondria
to generate energy and macromolecules for cell growth, is directly regulated by mTORC1. …

Macroautophagy is dispensable for growth of KRAS mutant tumors and chloroquine efficacy

…, G McAllister, GR Hoffman… - Proceedings of the …, 2016 - National Acad Sciences
Macroautophagy is a key stress-response pathway that can suppress or promote tumorigenesis
depending on the cellular context. Notably, Kirsten rat sarcoma (KRAS)-driven tumors …

Two-level total disc replacement with Mobi-C cervical artificial disc versus anterior discectomy and fusion: a prospective, randomized, controlled multicenter clinical …

…, MS Hisey, RJ Jackson, HW Bae, GA Hoffman… - … of Neurosurgery: Spine, 2015 - thejns.org
OBJECT The purpose of this study was to evaluate the safety and effectiveness of 2-level
total disc replacement (TDR) using a Mobi-C cervical artificial disc at 48 months' follow-up. …