Article Figures & Data
Figures
- Fig. 1
Live/Dead Staining of human mesenchymal stem cells after vancomycin exposure. Increased death of human mesenchymal stem cells was observed after exposure to 6400 μg/ml of vancomycin as compared to control. Live cells are stained in green while dead cells are stained in red.
- Fig. 2
Functional assessment of human mesenchymal stem cells exposed to vancomycin. Alizarin Red staining was used to assess the function of MSCs that were previously exposed to 24 hours of vancomycin. Red staining indicates the presence of calcium deposition. While statistically significant cell death was seen at all tested doses, calcium deposition appeared to drop dramatically at the highest dose of vancomycin tested.
Tables
Vancomycin Concentration (μg/mL) % Cell Death compared to 0 μg/mL p-Value 0 (control) --- 400 9.43% 0.047 1600 13.79% 0.0047 3200 19.35% <0.0001 4800 13.58% 0.0043 6400 24.82% <0.0001 12800 51.83% <0.0001 Statistically significant cell death was seen at all tested doses of vancomycin. Increased doses of of vancomycin were associated with increased cell death.
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