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Research ArticleBIOLOGICS
Open Access

Co-culture of Adult Mesenchymal Stem Cells and Nucleus Pulposus Cells in Bilaminar Pellets for Intervertebral Disc Regeneration

Aliza A. Allon, Richard A. Schneider and Jeffrey C. Lotz
International Journal of Spine Surgery January 2009, 3 (2) 41-49; DOI: https://doi.org/10.1016/SASJ-2009-0005-NT
Aliza A. Allon
Department of Orthopaedic Surgery, University of California, San Francisco, California, USA
MS
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Richard A. Schneider
Department of Orthopaedic Surgery, University of California, San Francisco, California, USA
Ph.D
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Jeffrey C. Lotz
Department of Orthopaedic Surgery, University of California, San Francisco, California, USA
Ph.D
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Figures

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  • Figure 1
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    Figure 1

    The formation of co-culture pellets.

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    Figure 2

    Co-culture pellets budding and forming satellites.

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    Figure 3

    Frozen and paraffin histological sections.

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    Figure 4

    The main pellet maintains structure as satellites break off.

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    Figure 5

    Satellite pellets exhibit the same structure: MSC inside and NPC outside.

Tables

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    Table 1

    Top primary inpatient spine surgeries where DDD is the primary diagnosis (under 65)

    ICD-9 procedure codeDefinition%
    81.08Posterior lumbar fusion52.50%
    81.06Anterior lumbar fusion27.27%
    3.09Decompression of the spinal canal4.80%
    81.07Lateral lumbar fusion4.67%
    80.51Discectomy4.22%
    Others6.54%
    Total100.00%
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    Table 2

    Degenerative Disc Disease Diagnosis by Age & Gender, United States, 2004-2007

    MalesFemales
    Age groupTotal% of diagnoses by age under 65 (Males)Total% of diagnoses by age under 65 (Females)
    <140.00%40.0%
    130.00%10.0%
    2--4110.01%50.0%
    59240.02%210.0%
    10141730.12%1840.1%
    15-191,1510.79%1,1830.7%
    20-242,2411.53%2,5271.5%
    25-295,5733.81%5,6863.3%
    30-3410,2206.99%9,6755.6%
    35-3915,31710.48%14,8628.5%
    40-4419,47813.33%21,21112.2%
    45-4923,82316.30%28,12716.2%
    50-5424,78116.95%31,98518.4%
    55-5923,36215.98%31,56418.1%
    60-6420,01013.69%26,91515.5%
    146,171173,950
    • Source: PearlDiver data derived from HIPAA-compliant insurance and private payer sources. These tables and charts are for information purposes only. The PearlDiver data has been obtained or derived from sources believed by PearlDiver to be reliable, but PearlDiver Technologies Inc. does not represent that the information is 100% accurate or complete. Any opinions or estimates which form any portion of the tables, charts or other aspects of this report represent the judgment of PearlDiver's analysts and management and are subject to change without notice. Copyright © 2009 PearlDiver Technologies Inc.

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International Journal of Spine Surgery
Vol. 3, Issue 2
1 Jan 2009
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Co-culture of Adult Mesenchymal Stem Cells and Nucleus Pulposus Cells in Bilaminar Pellets for Intervertebral Disc Regeneration
Aliza A. Allon, Richard A. Schneider, Jeffrey C. Lotz
International Journal of Spine Surgery Jan 2009, 3 (2) 41-49; DOI: 10.1016/SASJ-2009-0005-NT

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Co-culture of Adult Mesenchymal Stem Cells and Nucleus Pulposus Cells in Bilaminar Pellets for Intervertebral Disc Regeneration
Aliza A. Allon, Richard A. Schneider, Jeffrey C. Lotz
International Journal of Spine Surgery Jan 2009, 3 (2) 41-49; DOI: 10.1016/SASJ-2009-0005-NT
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Keywords

  • co-culture
  • bilaminar structure
  • Intervertebral disc
  • nucleus replacement
  • tissue engineering

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