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Research ArticleLumbar Spine

Locally Delivered Ascorbic Acid and β-Glycerophosphate Augment Local Bone Graft in a Murine Model of 2-Level Posterior Spinal Fusion

Joshua Vic Chen, Katie Lee, Kyle Tillinghast, Bernard Halloran and Alan B.C. Dang
International Journal of Spine Surgery October 2021, 15 (5) 921-928; DOI: https://doi.org/10.14444/8120
Joshua Vic Chen
1Department of Orthopaedic Surgery, University of California, San Francisco, California
BA
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Katie Lee
1Department of Orthopaedic Surgery, University of California, San Francisco, California
BA
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Kyle Tillinghast
1Department of Orthopaedic Surgery, University of California, San Francisco, California
MD
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Bernard Halloran
2Orthopaedic Section, Department of Surgery, San Francisco VA Health Center, San Francisco, California
PhD
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Alan B.C. Dang
1Department of Orthopaedic Surgery, University of California, San Francisco, California
2Orthopaedic Section, Department of Surgery, San Francisco VA Health Center, San Francisco, California
MD
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  • Figure 1
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    Figure 1

    Axial micro-CT images (ascorbic acid group). The border between native lamina and newly formed bone is poorly defined, requiring the referencing of the anterior vertebral body to assess bone volume.

  • Figure 2
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    Figure 2

    Analysis was performed on micro-CT images where there were continuous vertebral lamina, pedicles, and spinous processes, ensuring consistent anterior vertebral body sampling between levels. (Top) Images with noncontinuous vertebral lamina, pedicle, or spinous process. (Bottom) Images used for reference calculation.

  • Figure 3
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    Figure 3

    (Top) A line is drawn connecting the dorsal-most point of the vertebral foramen to the dorsal-most point of the vertebral body. (Middle) A bisecting perpendicular line is drawn, separating the dorsal from the ventral portion. (Bottom) Example dorsal regions of interest are traced.

  • Figure 4
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    Figure 4

    A line of least distance bridges noncontinuous cortical bone, drawn over a micro–computed tomography image (ascorbic acid group). The circular region of interest in the middle image identifies the noncontinuous cortical bone.

  • Figure 5
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    Figure 5

    Corrected bone volume (cBV) is statistically significantly increased in the ascorbic acid (VIT-C) group compared with the control group (P < .0001), and in the VIT-C group compared with the sponge group (P = .0360). The difference in cBV between the sponge group and control group is nonsignificant.

  • Figure 6
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    Figure 6

    Mineralized bone fraction is statistically significantly decreased when comparing the VIT-C group to both the control group and sponge group (P = .0421; P = .0078). The difference in mineralized bone fraction between the sponge group and control group is nonsignificant.

  • Figure 7
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    Figure 7

    There is a nonsignificant difference in fusion rate between any test group. Fusion rate is nonsignificantly increased when comparing both the sponge group and ascorbic acid (VIT-C) group to the control group (P = .3911; P = .2743).

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International Journal of Spine Surgery
Vol. 15, Issue 5
1 Oct 2021
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Locally Delivered Ascorbic Acid and β-Glycerophosphate Augment Local Bone Graft in a Murine Model of 2-Level Posterior Spinal Fusion
Joshua Vic Chen, Katie Lee, Kyle Tillinghast, Bernard Halloran, Alan B.C. Dang
International Journal of Spine Surgery Oct 2021, 15 (5) 921-928; DOI: 10.14444/8120

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Locally Delivered Ascorbic Acid and β-Glycerophosphate Augment Local Bone Graft in a Murine Model of 2-Level Posterior Spinal Fusion
Joshua Vic Chen, Katie Lee, Kyle Tillinghast, Bernard Halloran, Alan B.C. Dang
International Journal of Spine Surgery Oct 2021, 15 (5) 921-928; DOI: 10.14444/8120
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  • Safety and Viability of Anterior Lumbar Interbody Fusion in Complex Revision Lumbar Spine Surgeries: Insights From a Case Series of 135 Patients on Transforaminal Lumbar Interbody Fusion/Posterior Lumbar Interbody Fusion Cage Removal
  • Canal Bone Ratio for Predicting Bone Mineral Density in Lumbar Degenerative Diseases
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Keywords

  • ascorbic acid
  • β-glycerophosphate
  • bone graft enhancer
  • spinal fusion
  • bone mineral density

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