Research ArticleBiologics

Intradiscal Delivery of Anabolic Growth Factors and a Metalloproteinase Inhibitor in a Rabbit Acute Lumbar Disc Injury Model

SAPAN D. GANDHI, TRISTAN MAERZ, SEAN MITCHELL, CASEY BACHISON, DANIEL K. PARK, JEFFREY S. FISCHGRUND and KEVIN C. BAKER
International Journal of Spine Surgery August 2020, 14 (4) 585-593; DOI: https://doi.org/10.14444/7078

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Figures

  • Figure 1
    Figure 1

    Quantitative results of differentiation assays. (A) Both chondrogenic media and experimental media containing TGF-β3, BMP-4, and TIMP-2 induced time-dependent increases in Alcian blue staining. (B) There was significantly greater staining intensity compared with control P2-3 cells treated with basal media.

  • Figure 2
    Figure 2

    Normalized nucleus pulposus (NP)-only T2 relaxation times of defect-only discs, and discs treated with chitosan alone, chitosan containing TGF-β3 and BMP-4, or chitosan containing TGF-β3, BMP-4 and TIMP-2. *Significant difference between treated and defect-only discs at P < .05.

  • Figure 3
    Figure 3

    Quantitative analysis of Safranin-O staining intensity (A) and total disc size (B) from Safranin-O/Fast Green–stained decalcified sections. *Significant difference between treatment and defect discs.

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Intradiscal Delivery of Anabolic Growth Factors and a Metalloproteinase Inhibitor in a Rabbit Acute Lumbar Disc Injury Model
SAPAN D. GANDHI, TRISTAN MAERZ, SEAN MITCHELL, CASEY BACHISON, DANIEL K. PARK, JEFFREY S. FISCHGRUND, KEVIN C. BAKER
International Journal of Spine Surgery Aug 2020, 14 (4) 585-593; DOI: 10.14444/7078
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